Hydrophobic binding of ribosomes and polysomes to agarose gels

✍ Scribed by S.L. Petrovic´; M.B. Novakovic´; R.M. Metlasˇ

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 545 KB
• Volume: 90
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90039-8

✦ Synopsis

Rat liver ribosomes and polyribosomes could be immobilized in agarose gels at 4°C and pH 7.6, using KC1 or NaCl molarities of 0.25 or higher. The binding could be effected in the presence of excess protein and/or detergents. Polysomes attached to endoplasmic membrane fragments did not bind to agarose even at 0.5 M KCI; tRNAs were also not bound. The larger (60 S) subunit of liver ribosomes was also completely immobilized at 0.3 M KCI, while the immobilization of the smaller (40 S) subunit was poor even at 1 M KCI. The ribosomal subunits could be essentially quantitatively desorbed at 4°C by a low ionic strength elution, while the recovery of gel-bound polysomes was of the order of 80 to 85% under these conditions. The polysomes that recovered from agarose at low ionic strength were active in in vitro incorporation of amino acids into polypeptides.