𝔖 Bobbio Scriptorium
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Reversed micelles recognize an active protein

✍ Scribed by Masahiro Goto; Tsutomu Ono; Fumiyuki Nakashio; T. Alan Hatton

Book ID
104648441
Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
258 KB
Volume
10
Category
Article
ISSN
0951-208X

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✦ Synopsis

The extraction behavior of native and heated-denatured a-chymatrypsin has been investigated with two different reversed micellar systems. A large difference in the degree of extraction was observed for the native relative to the denatured a-chymotrypsin. In particular, mixed reversed micelles formulated with DOLPA (dioleyl phosphoric acid) and AOT show a high selectivity for the active ol-chymotrypsin.

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