A rapid and simple reverse-phase high performance liquid chromatographic method has been developed for the quantitative determination of asiaticoside, a pharmacologically active constituent from Centella asiatica. Using an octadecyl silane-packed column eluted with water (containing 1% trifluoroacet
Reverse-phase High Performance Liquid Chromatography of Sennosides in Cassia angustifolia
✍ Scribed by R. K. Verma; G. C. Uniyal; S. P. Singh; J. R. Sharma; M. M. Gupta
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 203 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0958-0344
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✦ Synopsis
A rapid and simple reverse-phase high performance liquid chromatographic method has been developed for the quantitative estimation of the medicinally useful laxative principles sennoside A and sennoside B in senna. Using a Bondapak C,, column eluted with methano1:water:acetic acid:tetrahydrofuran (60:38:2:2) both compounds were well resolved with recoveries of 97 and 96% respectively.
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