Capillary reverse-phase high-performance liquid chromatography (HPLC) with electrospray condensation particle counting detection

✍ Scribed by Kenneth C. Lewis; James W. Jorgenson; Stanley L. Kaufman; Jeff W. Skogen

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 116 KB
• Volume: 10
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1998)10:6<467::aid-mcs1>3.0.co;2-e

✦ Synopsis

Protein standards are separated on a reverse-phase liquid chromatography column with 40 m inner diameter. They are introduced to the gas phase by micro-electrospray and detected by a condensation particle counter. The microelectrospray needle is a new design which allows for the use of a nanoliter-perminute makeup flow instead of the conventional microliter-per-minute flow. The observed separation efficiency is very good and the sheath micro-electrospray needle performs flawlessly while the system detection limit is comparable to on-column UV absorption.