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Reverse-phase high performance liquid chromatography of asiaticoside in Centella asiatica

✍ Scribed by R. K. Verma; K. G. Bhartariya; M. M. Gupta; Sushil Kumar

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
48 KB
Volume
10
Category
Article
ISSN
0958-0344

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✦ Synopsis

A rapid and simple reverse-phase high performance liquid chromatographic method has been developed for the quantitative determination of asiaticoside, a pharmacologically active constituent from Centella asiatica. Using an octadecyl silane-packed column eluted with water (containing 1% trifluoroacetic acid):methanol (30:70, v/v), asiaticoside was well resolved from other constituents. These optimized analytical conditions allowed the determination of asiaticoside with a recovery of 97%.

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