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Restriction map of the hepatitis B virus DNA cloned in Escherichia coli

✍ Scribed by Bichko, V.V.; Kozlovskaya, T.M.; Dishler, A.; Pumpen, P.; Janulaitis, A.; Gren, E.J.


Book ID
122730887
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
267 KB
Volume
20
Category
Article
ISSN
0378-1119

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## Abstract DNA from the ayw subtype of hepatitis B virus (HBV) was ligated into the EcoRI site of DNA from plasmid pBR322 and propagated in E coli Ο‡1776. A plasmid with a 3200 base pair insert (pHBV‐1) was isolated and the cloned HBV DNA was mapped with restriction endonucleases. Differences were