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Mapping, cloning, and DNA sequencing of pepB which encodes peptidase B of Escherichia coli K-12

โœ Scribed by Hideyuki Suzuki; Eun-Soo Kim; Nagi Yamamoto; Wataru Hashimoto; Kenji Yamamoto; Hidehiko Kumagai


Book ID
113397957
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
776 KB
Volume
82
Category
Article
ISSN
0922-338X

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Using lambda phage clones containing segments of the Escherichia coli K12 chromosome as hybridization probes, we found one gene at 42 min on the E. coli chromosome map, the expression of which was affected by RNase III. The sequence of the DNA fragment containing this gene (gen-165) revealed the pre