Restriction enzyme mapping of a bacteriophage lambda DNA
β Scribed by Noreen Cunningham; James Tomlinson; Frances Jurnak
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 446 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0307-4412
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## Abstract A highβresolution plotter of differential melting profiles of DNA, RNA, or related biopolymers with an onβline miniβcomputer is described. With this device, more than 15 transition steps were identified in the thermal melting profile of DNA from bacteriophage lambda. These fine structur
The cytosine-containing DNA of a mutant of bacteriophage T4 was digested with restriction endonucleases SmaI, KpnI and BglII producing 5, 7 and 13 fragments respectively. Complete physical maps of the T4 genome were constructed with the enzymes SmaI and KpnI and an almost complete map with the enzym