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Hyperfine structure in melting profile of bacteriophage lambda DNA

✍ Scribed by Osamu Gotoh; Yuzuru Husimi; Sadato Yabuki; Akiyoshi Wada

Publisher: Wiley (John Wiley & Sons)
Year: 1976
Tongue: English
Weight: 764 KB
Volume: 15
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1976.360150406

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✦ Synopsis

Abstract

A high‐resolution plotter of differential melting profiles of DNA, RNA, or related biopolymers with an on‐line mini‐computer is described. With this device, more than 15 transition steps were identified in the thermal melting profile of DNA from bacteriophage lambda. These fine structures were found to be reproducible, and some of them disappear in the deletion mutant.

To Examine the melting profile, computer simulations for several hypothetical polynucleotide sequences were performed, and compared with experimental data. The sharp peaks that appeared in the differential melting profile of λ DNA may come from some homogeneous sequences of 500 bases or longer.

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