Determination of DNA base compositions from melting profiles in dilute buffers

✍ Scribed by R. J. Owen; L. R. Hill; S. P. Lapage

• Publisher: Wiley (John Wiley & Sons)
• Year: 1969
• Tongue: English
• Weight: 628 KB
• Volume: 7
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1969.360070408

✦ Synopsis

Equations were determined for the dependency of the melting temperature (TJ of DNA upon the logarithm of the sodium ion concentration, for four DNA samples of widely diflerent base compositions (eoc). The dopee of these T, versus log M equations were found to decrease with increasing Boc of the samples. An empirical equation relating T, , log M (Na+) and eoc was derived, which also accounts for merences in T, v e m s log M slopes. Data from the literature for some synthetic polynucleotides and for the crab (Cancer puguru-s) satellite poly AT are discussed in relation to the above finding. The changes in T, versus log M slopes with Boc are interpreted in terms of changes in the thermodynamic parameters A S and A H with base compwition.

* For brevity, equations for the dependency of T, upon log M @a+) (eoc constant) will be called T, versus log M equations; similarly, equations for the dependency of T, upon Boc [log M (Na+) constant] will be called T, versus 6GC equations.