Rolling circle replicative structure of bacteriophage λ DNA in a recombination deficient system

✍ Scribed by Takahashi, Seishi

• Publisher: Springer
• Year: 1977
• Tongue: English
• Weight: 334 KB
• Volume: 152
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00268818

✦ Synopsis

Rolling-circle replicating structures which represent late stage lambda DNA replication can be detected among intracellular phage lambda DNA molecules under recombination deficient conditions as well as in wild-type infections. Furthermore, if initiation of lambda replication is delayed until the late stage of lambda infection, then nearly all replicating molecules are rolling-circle, even in the first round. Thus neither genetic recombination nor termination of a round of replication are required for generation of rolling-circle replicating molecules of lambda DNA.