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Physical mapping of the restriction fragments obtained from bacteriophage T4 dC-DNA with the restriction endonucleases SmaI, KpnI and BglII

✍ Scribed by Kiko, Heinz ;Niggemann, Elisabeth ;Rüger, Wolfgang

Publisher: Springer
Year: 1979
Tongue: English
Weight: 806 KB
Volume: 172
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00271730

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✦ Synopsis

The cytosine-containing DNA of a mutant of bacteriophage T4 was digested with restriction endonucleases SmaI, KpnI and BglII producing 5, 7 and 13 fragments respectively. Complete physical maps of the T4 genome were constructed with the enzymes SmaI and KpnI and an almost complete map with the enzyme BglII.

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The complete maps of BglII, SalI and Xho

The complete maps of BglII, SalI and XhoI restriction sites on T4 dC-DNA

✍ Rüger, Wolfgang ;Neumann, Manfred ;Rohr, Ute ;Niggemann, Elisabeth 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 856 KB

T4 dC-DNA was digested with the restriction endonucleases BglII, SalI and XhoI. Overlaps in the three sets of fragments allowed the mapping of all restriction sites relative to each other along the T4 genome.