A general method for the transfer of pla
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Meulenberg, J. J. M. ;Loenen, W. A. M. ;Sellink, E. ;Postma, P. W.
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Article
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1990
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Springer
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English
⚖ 364 KB
A generally applicable method is described for reintroduction of mutant plasmid-borne alleles to the chromosome of Klebsiella pneumoniae using bacteriophage 2. We,used this method to make stable chromosomal transposon insertions in genes for biosynthesis of pyrroloquinoline quinone in K. pneumoniae