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Replication origin of the Bacillus subtilis chromosome determined by hybridization of the first-replicating DNA with cloned fragments from the replication origin region of the chromosome

โœ Scribed by Naotake, Ogasawara; Sumi, Mizumoto; Hiroshi, Yoshikawa

Book ID
123298582
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
841 KB
Volume
30
Category
Article
ISSN
0378-1119

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A BamHI restriction endonuclease fragment B7, which contains the replication origin of the Bacillus subtilis chromosome, showed inhibitory effects on cell growth and plasmid replication in Escherichia coli and Bacillus subtilis, when B7 was inserted into a composite plasmid pMS102' and introduced in