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Ribosomal RNA genes in the replication origin region of Bacillus subtilis chromosome

✍ Scribed by Henckes, G.; Vannier, F.; Seiki, M.; Ogasawara, N.; Yoshikawa, H.; Seror-Laurent, S. J.


Book ID
109727580
Publisher
Nature Publishing Group
Year
1982
Tongue
English
Weight
451 KB
Volume
299
Category
Article
ISSN
0028-0836

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✍ Seiki, Motoharu ;Ogasawara, Naotake ;Yoshikawa, Hiroshi πŸ“‚ Article πŸ“… 1981 πŸ› Springer 🌐 English βš– 791 KB

A BamHI restriction endonuclease fragment, B7, which is replicated first among all other fragments derived from the Bacillus subtilis chromosome, was cloned in Escherichia coli using as vector a hybrid plasmid pMS102 that can replicate both in E. coli and B. subtilis. Digestion of pMS102 with BamHI

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A BamHI restriction endonuclease fragment B7, which contains the replication origin of the Bacillus subtilis chromosome, showed inhibitory effects on cell growth and plasmid replication in Escherichia coli and Bacillus subtilis, when B7 was inserted into a composite plasmid pMS102' and introduced in