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Structure of the region of the replication origin of the Bacillus subtilis chromosome

โœ Scribed by Seiki, Motoharu; Ogasawara, Naotake; Yoshikawa, Hiroshi

Book ID
109716494
Publisher
Nature Publishing Group
Year
1979
Tongue
English
Weight
421 KB
Volume
281
Category
Article
ISSN
0028-0836

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๐Ÿ“œ SIMILAR VOLUMES

Structure and function of the region of
Structure and function of the region of the replication origin of the Bacillus subtilis chromosome
โœ Seiki, Motoharu ;Ogasawara, Naotake ;Yoshikawa, Hiroshi ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 791 KB

A BamHI restriction endonuclease fragment, B7, which is replicated first among all other fragments derived from the Bacillus subtilis chromosome, was cloned in Escherichia coli using as vector a hybrid plasmid pMS102 that can replicate both in E. coli and B. subtilis. Digestion of pMS102 with BamHI

Structure and function of the region of
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โœ Seiki, Motoharu ;Ogasawara, Naotake ;Yoshikawa, Hiroshi ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 742 KB

A BamHI restriction endonuclease fragment B7, which contains the replication origin of the Bacillus subtilis chromosome, showed inhibitory effects on cell growth and plasmid replication in Escherichia coli and Bacillus subtilis, when B7 was inserted into a composite plasmid pMS102' and introduced in