Genes and their organization in the replication origin region of the Bacillus subtilis chromosome: Comparison with the Escherichia coli chromosome
β Scribed by Ogasawara, N
- Book ID
- 122836801
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 789 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0065-227X
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A BamHI restriction endonuclease fragment, B7, which is replicated first among all other fragments derived from the Bacillus subtilis chromosome, was cloned in Escherichia coli using as vector a hybrid plasmid pMS102 that can replicate both in E. coli and B. subtilis. Digestion of pMS102 with BamHI
A BamHI restriction endonuclease fragment B7, which contains the replication origin of the Bacillus subtilis chromosome, showed inhibitory effects on cell growth and plasmid replication in Escherichia coli and Bacillus subtilis, when B7 was inserted into a composite plasmid pMS102' and introduced in