F' strains of E. coli have been isolated which are merodiploid for various chromosomal segments between 66 and 78 minutes. Strains diploid for the chromosomal DNA between the genes bgl and mtl grow slowly, have a reduced DNA/mass and an increased cell size. These properties could result if the chrom
Methylation strongly enhances DNA bending in the replication origin region of the Escherichia coli chromosome
β Scribed by Kimura, Toru ;Asai, Tsuneaki ;Imai, Mutsuo ;Takanami, Mituru
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 886 KB
- Volume
- 219
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
After initiation of chromosome replication in Escherichia coli, the newly replicated, hemi-methylated origins of replication are exempt from both re-initiation and methylation for up to a third of the generation time. In the model proposed here, the exemption from methylation is attributed to the ph
A precise genetic-physical map of the tnailv region at 82 rain on the genetic map of E. coli is obtained through deletion mapping and analysis by restriction endonuclease EcoRI of plasmids, derived from an F ~ carrying the genes between aroE and ilv. A locus, designated het, which in its diploid st
It has been found that strains carrying mutations in the dnaA gene are unusually sensitive to COU, NAL or NOV, which are known to inhibit DNA gyrase activities. The delay in the initiation of chromosome replication after COU treatment has been observed in cells with chromosomes synchronized by amino
## Abstract **Background:** During the cell cycle, the initiation of chromosomal replication is strictly controlled. In __Escherichia coli__, the initiator DnaA and the replication origin __oriC__ are major targets for this regulation. Here, we assessed the role of transcription of the __mioC__ gen