Renal-cell carcinoma-specific lysis by cytotoxic T-lymphocyte clones isolated from peripheral blood lymphocytes and tumor-infiltrating lymphocytes

✍ Scribed by Nathalie Brouwenstijn; Béatrice Gaugler; K. Margaretha Krüse; Corry W. van der Spek; Arend Mulder; Susanne Osanto; Benoît J. van den Eynde; Peter I. Schrier

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: French
• Weight: 604 KB
• Volume: 68
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19961009)68:2<177::aid-ijc6>3.0.co;2-v

✦ Synopsis

Melanoma and renal-cell carcinoma (RCC) are generally consided to be relatively immunogenic tumor types in humans. In the case of melanoma, many major histocompatibility complex (MHC) class I-restricted tumor-specific cytotoxic T lymphocytes (Cn) have been isolated from either tumorinfiltrating lymphocytes (TIL) or autologous peripheral blood lymphocytes (PBL). In contrast, such CTL have only incidentally been described in the case of RCC. It has often been reported that TIL lines isolated from RCC display non-MHC-restricted and non-specific activity. Here, we report the isolation and characterization of tumor-specific CTL from PBL of one RCC patient and from TIL of another RCC patient. CTL clones 263/ I 7 and 263/45, isolated from the PBL of patient LE-92 I I, were restricted by HLA-67. CTL clone 5E, isolated from the TIL of patient LE-89 15, was restricted by HLA-B37. The autologous RCC cell lines were efficiently b e d by the Cn clones, whereas normal epithelial cells of the proximal tubuli matched for the restriction element and K562 were not. From a panel of allogeneic RCC cell lines, CTL 5E recognized MZ-1940-RCC. Reactivity to allogeneic RCC sharing HLA-B7 was also observed with CTL 263/17 and 263/45, both of which could l p the HLA-B7-positive cell line MZ-I85 I -RCC. Our data provide evidence that common tumor antigens are recognized by CTL on RCC.