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Remote Participation-Assisted Synthesis of β-Mannosides.

✍ Scribed by Cristina De Meo; Medha N. Kamat; Alexei V. Demchenko

Publisher: John Wiley and Sons
Year: 2005
Weight: 35 KB
Volume: 36
Category: Article
ISSN: 0931-7597
DOI: 10.1002/chin.200526127

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Engineering enzymes: The glutamic acid nucleophile of a retaining β-mannosidase has been replaced with a serine residue to form a β-mannosynthase. When the new enzyme is provided with an α-mannosyl fluoride donor and an appropriate acceptor, β-mannoside linkages are synthesized. Remarkably, α-mannos