An Aspergillus nidulans gene library was constructed in a high-frequency transformation vector, pDJB3, based on the Neurospora crassa pyr4 gene. This gene library was used to isolate the structural gene for isocitrate lyase (acuD) by complementation of a deficiency mutation following transformation
Regulation of the expression of the isocitrate lyase gene (acuD) of Aspergillus nidulans
✍ Scribed by Bowyer, Paul ;De Lucas, J. Ramón ;Turner, Geoffrey
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 810 KB
- Volume
- 242
- Category
- Article
- ISSN
- 0026-8925
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The nucleotide sequences of the genes encoding the acetate-inducible glyoxylate cycle enzyme isocitrate lyase from the ascomycete fungi Aspergillus nidulans (acuD) and Neurospora crassa (acu-3) are presented. The respective A. nidulans and N. crassa genes are interrupted at identical positions by tw
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