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Gene cloning in Aspergillus nidulans: isolation of the isocitrate lyase gene (acuD)

✍ Scribed by Ballance, D. J. ;Turner, G.

Publisher: Springer
Year: 1986
Tongue: English
Weight: 676 KB
Volume: 202
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00331649

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✦ Synopsis

An Aspergillus nidulans gene library was constructed in a high-frequency transformation vector, pDJB3, based on the Neurospora crassa pyr4 gene. This gene library was used to isolate the structural gene for isocitrate lyase (acuD) by complementation of a deficiency mutation following transformation of A. nidulans. Plasmids rescued in Escherichia coli were able to transform five different A. nidulans acuD mutants. Transformation using plasmids containing the cloned fragment resulted in integration at the acuD locus in six of nine transformants.

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