Characterization of the glyoxysomal isocitrate lyase genes ofAspergillus nidulans (acuD)andNeurospora crassa (acu-3)
✍ Scribed by L. D. S. Gainey; I. F. Connerton; E. H. Lewis; G. Turner; D. J. Ballance
- Publisher
- Springer-Verlag
- Year
- 1992
- Tongue
- English
- Weight
- 615 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0172-8083
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✦ Synopsis
The nucleotide sequences of the genes encoding the acetate-inducible glyoxylate cycle enzyme isocitrate lyase from the ascomycete fungi Aspergillus nidulans (acuD) and Neurospora crassa (acu-3) are presented. The respective A. nidulans and N. crassa genes are interrupted at identical positions by two introns and encode proteins of 538 and 543 amino acids, which have 75% identity. The predicted protein sequences do not demonstrate the C-terminal tripeptide S-K-L that has been implicated in peroxisomal targeting and found in the glyoxysomally located enzyme malate synthase from the same species. However, the protein sequences do exhibit a partial repeat which, in common with malate synthase, is located in regions that are absent from, or non-homologous with, the E. coli enzyme, which is not compartmentalized.