Genetic regulation of isocitrate lyase in the yeastYarrowia lipolytica
β Scribed by Gerold Barth
- Publisher
- Springer-Verlag
- Year
- 1985
- Tongue
- English
- Weight
- 440 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0172-8083
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Thirteen chromosomal loci have been identified which affect acetate metabolism in Coprinus. Mutants at only two loci, acu-l and acu-7, are deficient in isocitrate lyase (ICL) (EC 4.1.3.1) activity. acu-1 mutants are unable to induce ICL because they lack acetyl-CoA synthetase which is required to co
The ICL1 gene encoding the isocitrate lyase from Saccharomyces cerevisiae was cloned and sequenced. A reading frame of 557 amino acids showing significant similarity to isocitrate lyases from seven other species could be identified. Construction of icl1 null mutants led to growth defects on C2 carbo
Studies on acetate utilization by Rhodopseudomonas capsutata strain St. Louis indicated that the wild type grew poorly on acetate and made little if any of the glyoxylate cycle enzyme isocitrate lyase. A spontaneous mutant, Ac-l, capable of vigorous and immediate growth on acetate and exhibiting hig