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Regulation of RNA synthesis in yeast III

✍ Scribed by Elliott, Steven G. ;McLaughlin, Calvin S.

Publisher: Springer
Year: 1979
Tongue: English
Weight: 573 KB
Volume: 169
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00382269

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✦ Synopsis

Centrifugal elutriation was used to separate cells in different stages of the cell cycle from a culture of Saccharomyces cerevisiae in balanced exponential growth. The rate of DNA and RNA synthesis was determined using a pulse -long-term label technique that is capable of distinguishing between exponential, linear, and periodic variations in the rate of synthesis through the cell cycle. It was found that while the rate of DNA synthesis varies periodically through the cell cycle, the rate of synthesis of mRNA, rRNA, and tRNA increases exponentially through the cell cycle. The implications of these findings for the control of RNA synthesis are discussed.

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