The stringent control of RNA synthesis in the yeast Saccharomyces cerevisiae may be evoked either by starving for a required amino acid or by inhibiting protein synthesis. The response is non-coordinate in that the synthesis of ribosomal and messenger RNA is depressed whereas that of transfer RNA co
Antisense RNA regulation of theILV2gene in yeast: a correction
โ Scribed by Greg M. Arndt; W. Xiao; G. H. Rank
- Publisher
- Springer-Verlag
- Year
- 1994
- Tongue
- English
- Weight
- 79 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0172-8083
No coin nor oath required. For personal study only.
โฆ Synopsis
A previous report of the use of antisense RNA to regulate gene expression in yeast is incorrect.
๐ SIMILAR VOLUMES
Centrifugal elutriation was used to separate cells in different stages of the cell cycle from a culture of Saccharomyces cerevisiae in balanced exponential growth. The rate of DNA and RNA synthesis was determined using a pulse -long-term label technique that is capable of distinguishing between expo
A study has been made of the regulation of the synthesis of Pl double-stranded (ds) RNA, the genome of the yeast virus-like particle. When yeast protein synthesis is prevented by starvation for a required amino acid or by addition of cycloheximide, the rate of Pl dsRNA synthesis is reduced markedly.
We report for the first time on the regulation of gene expression in yeast by antisense RNA. Chimaeric genes were constructed containing the 5' upstream and partial coding sequence of SMR1 -a sulfometuron methyl resistant allele of the IL V2 locus. Such fragments were placed 5' to 3' and 3' to 5' un
Antisense transcripts are typically associated with the down-regulation of gene expression. In this issue, Moorwood et al. present evidence that an antisense RNA can enhance expression of the Wilms' tumour suppressor locus WT1. We suggest that the unusual function of the WT1 antisense RNA might rela