Isolated microspores of Chinese cabbage (Brassica campestris ssp. pekinensis) were incubated in modified NN medium containing 10% sucrose in darkness at 33ยฐC for one day followed by culture at 25ยฐC. After 14 days of culture, microspores developed into embryos ranging from globular to cotyledonary st
Regeneration of fertile green plants from oat isolated microspore culture
โ Scribed by Parminder K. Sidhu; Philip A. Davies
- Publisher
- Springer
- Year
- 2009
- Tongue
- English
- Weight
- 340 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0721-7714
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Subculture regime and carbohydrate concentration of the medium had a marked effect on the regeneration of green plantlets from mechanically isolated microspores of Hordeum vulgare L. cv. Kymppi. A sevenfold increase in the yield of green plants was obtained by shortening the suspension culture time
Protoplasts were isolated from immature inflorescence-derived embryogenic suspension cultures of two cultivars of Sorghum vulgare. The protoplasts were cultured in a modified K8P liquid medium. They started to divide after 4-5 days of culture, and achieved 16.8% division frequency by 10 days. Protoc