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Plant regeneration from isolated microspore cultures of Chinese cabbage (Brassica campestrisspp.pekinensis)

✍ Scribed by Takanori Sato; Takeshi Nishio; Masashi Hirai

Publisher: Springer
Year: 1989
Tongue: English
Weight: 716 KB
Volume: 8
Category: Article
ISSN: 0721-7714
DOI: 10.1007/bf00269055

No coin nor oath required. For personal study only.

✦ Synopsis

Isolated microspores of Chinese cabbage (Brassica campestris ssp. pekinensis) were incubated in modified NN medium containing 10% sucrose in darkness at 33°C for one day followed by culture at 25°C. After 14 days of culture, microspores developed into embryos ranging from globular to cotyledonary stage. Plants were regenerated after transfer of embryos to medium containing 3% sucrose and no plant growth regulators.

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