Somatic embryogenesis and plant regeneration in cotyledonary explant cultures of Chinese cabbage

✍ Scribed by Pil S. Choi; Woong Y. Soh; Jang R. Liu

• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 558 KB
• Volume: 44
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00048532

✦ Synopsis

Cotyledonary explants of Chinese cabbage were cultured on Murashige and Skoog's medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid. Up to 20% of the cotyledonary explants produced somatic embryos with or without intervening callus production. Explants became more competent as the age of the source seedlings increased up to 8 days, but cotyledonary explants from 10-day-old seedlings were not responsive. Upon transfer to MS basal medium most of the somatic embryos developed into plantlets. Plantlets were transplanted to potting soil and grown to maturity in a phytotron. Among three cultivars used, only cotyledonary explants of 'Top Salad' were capable of producing somatic embryos.