๐”– Bobbio Scriptorium
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Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinumL.)

โœ Scribed by K. S. Barna; A. K. Wakhlu

Publisher
Springer
Year
1993
Tongue
English
Weight
624 KB
Volume
12
Category
Article
ISSN
0721-7714

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โœฆ Synopsis

Somatic embryogenesis and plant regeneration were obtained from immature leaflet callus of chickpea. Numerous globular embryos developed on the surface of callus on Murashige and Skoog's (1962) medium containing 25 ฮผM 2,4-dichlorophenoxyacetic acid. These globular embryos differentiated into mature somatic embryos upon removal of 2,4-dichlorophenoxyacetic acid. The maturation of embryos was significantly affected by pH, photoperiod, abscisic acid and genotype. Callus continued to produce somatic embryos for over 8 subcultures at 4 week intervals. Two per cent of the embryos formed plants on medium containing 15 ฮผM gibberellic acid and 1 ฮผM indole-3-butyric acid. Desiccation of embryos for a period of 3 d increased their rate of conversion into plants from 0.9 to 2.8%. All regenerated plants showed normal morphological characteristics.

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