✦ LIBER ✦

Plant regeneration through somatic embryogenesis from spear callus culture ofAsparagus cooperiBaker

✍ Scribed by Biswajit Ghosh; Sumitra Sen

Publisher: Springer
Year: 1991
Tongue: English
Weight: 985 KB
Volume: 9
Category: Article
ISSN: 0721-7714
DOI: 10.1007/bf00235353

No coin nor oath required. For personal study only.

✦ Synopsis

Somatic embryogenesis and plantlet formation were obtained from callus derived from the subapical region of spears of Asparagus cooperi Baker. Callus was obtained in Murashige and Skoog's medium supplemented with 1-naphthaleneacetic acid and kinetin. Increase in the concentration of potassium nitrate in subsequent subcultures resulted in the formation of embryos. Rapid multiplication of embryos was secured on transfer to a medium containing a different source of nitrogen and a low level (0.01 mg/1) of gibberellic acid. Media containing zeatin or gibberellic acid led to the formation of complete plantlets from embryos. Regenerated plants were cytologically and phenotypically stable.

📜 SIMILAR VOLUMES

Somatic embryogenesis and plant regenera

Somatic embryogenesis and plant regeneration from callus cultures ofBunium persicumBoiss

✍ A. K. Wakhlu; Sangita Nagari; K. S. Barna 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 492 KB

Callus was obtained from mericarps of Bunium persicum Boiss. on MS medium supplemented with 2.0 mg/1 2,4-D and 4.0 mg/1 Kn. Small white clumps of compactly packed cells developed on the callus on a medium containing 1.0 mg/1 2,4-D and 0 mg/1 Kn. These cell clumps differentiated numerous globular emb

Plant regeneration by somatic embryogene

Plant regeneration by somatic embryogenesis from callus cultures of sweet sorghum

✍ Christy MacKinnon; Glenys Gunderson; Murray W. Nabors 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 640 KB

Plant regeneration through somatic embry

Plant regeneration through somatic embryogenesis in callus culture of green bamboo (Bambusa oldhamiiMunro)

✍ Meei ling Yeh; Wei chin Chang 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 816 KB

Somatic embryogenesis and plant regenera

Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinumL.)

✍ K. S. Barna; A. K. Wakhlu 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 624 KB

Somatic embryogenesis and plant regeneration were obtained from immature leaflet callus of chickpea. Numerous globular embryos developed on the surface of callus on Murashige and Skoog's (1962) medium containing 25 μM 2,4-dichlorophenoxyacetic acid. These globular embryos differentiated into mature

High frequency somatic embryogenesis and

High frequency somatic embryogenesis and plant regeneration from zygotic embryo-derived callus cultures of threeAlliumspecies

✍ P. Valk; O. E. Scholten; F. Verstappen; R. C. Jansen; J. J. M. Dons 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 1004 KB

The plant regeneration ability of zygotic embryo-derived callus cultures was studied for 12 A. cepa varieties and accessions, two A. fistulosum varieties, one A. fistulosum x A. cepa interspecific hybrid and two A. porrum varieties. Compact embryogenic callus was induced on Murashige and Skoog (MS)

Rapid plant regeneration through organog

Rapid plant regeneration through organogenesis and somatic embryogenesis from cultured protoplasts ofBrassica juncea

✍ P. B. Kirti; V. L. Chopra 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 211 KB

Protoplasts derived from hypocotyls of seedlings grown on half-strength MS medium containing 1% sucrose were cultured at a density of 5 x 104 ml ~ in Kao's medium supplemented with 1.0 mg 1 ~ 2,4-D, 0.1 mg 1l NAA and 0.5mgl ~ zeatin riboside. After three days of culture in darkness at 25 + I°C, cult