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Culture conditions for efficient induction of green plants from isolated microspores of barley

✍ Scribed by Marjatta Salmenkallio-Marttila; Ulrika Kurtén; Veli Kauppinen

Publisher
Springer
Year
1995
Tongue
English
Weight
254 KB
Volume
43
Category
Article
ISSN
0167-6857

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✦ Synopsis

Subculture regime and carbohydrate concentration of the medium had a marked effect on the regeneration of green plantlets from mechanically isolated microspores of Hordeum vulgare L. cv. Kymppi. A sevenfold increase in the yield of green plants was obtained by shortening the suspension culture time of the developing proembryo mass from 4 to 3 weeks. A further twofold increase was obtained by increasing the maltose concentration of the microspore isolation medium and of the culture medium. Under optimal conditions, a mean of 169 + 97 green plants per spike were regenerated.

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