Rice cells were precultured for 10 d in medium containing 60 g/L sucrose and subsequently for 1 d in medium supplemented with 0. 4 M sorbitol. After loading with 25%PVS2 at 22ยฐC for 10 min and dehydration in 100%PVS2 at 0ยฐC for 7. 5 min,they were plunged into liquid nitrogen directly. Survival was 4
โฆ LIBER โฆ
Recovery of plants from cryopreserved embryogenic cell suspensions ofPinus caribaea
โ Scribed by Eric Laine; Pascale Bade; Alain David
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 1015 KB
- Volume
- 11-11
- Category
- Article
- ISSN
- 0721-7714
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