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Vitrificational cryopreservation and subsequently fertile plant regeneration from rice (Oryza sativaL.) embryogenic suspension cells

✍ Scribed by Junhui Wang; Yong Zheng; Qingfeng Yan; Qiusheng Yan; Xueqin Zhang; Chunnong Huang


Publisher
Springer
Year
1997
Tongue
English
Weight
290 KB
Volume
42
Category
Article
ISSN
1001-6538

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Plant regeneration from rice (Oryza sati
✍ Chun-Nong Huang; Jun-Hui Wang; Qiu-Sheng Yan; Xue-Qin Zhang; Qin-Feng Yan 📂 Article 📅 1995 🏛 Springer 🌐 English ⚖ 767 KB

Rice cells were precultured for 10 d in medium containing 60 g/L sucrose and subsequently for 1 d in medium supplemented with 0. 4 M sorbitol. After loading with 25%PVS2 at 22°C for 10 min and dehydration in 100%PVS2 at 0°C for 7. 5 min,they were plunged into liquid nitrogen directly. Survival was 4