Recognition of HLA-B27 by mouse cytotoxic T-cell clones: a transgenic mouse

✍ Scribed by Murielle Reboul; Bernard Frangoulis; Anna Rocca; Laurent Degos; Marika Pla

• Publisher: Springer-Verlag
• Year: 1991
• Tongue: English
• Weight: 476 KB
• Volume: 34
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00205824

✦ Synopsis

As a basis for the characterization of mouse T cells involved in the recognition of xenogeneic HLA molecules, a panel of HLA-B27-reactive cytotoxic T-cell clones was generated upon stimulation by cells from HLA-B27-transgenic mice. The HLA-B27-induced T-cell response was found to comprise two categories of clones: some recognizing HLA-B27 independent of H-2 molecules expressed by the target cells (unrestricted clones), others recognizing HLA-B27 in an H-2-restricted manner. The unrestricted clones exhibited diverse specificities, as judged from their various cross-reactivities with other xenogeneic (HLA) or allogeneic (H-2) molecules. In addition, although most of the unrestricted clones were able to react with both mouse and human HLA-B27-transgenic mice. The HLA-B27-induced T-cell which reacted only with HLA-B27-positive mouse, and not human cells. These findings illustrate that both H-2-restricted and unrestricted T cells with diverse specificities contribute to HLA-B27-xenorecognition.