Recognition of chlamydial antigen by HLA-B27-restricted cytotoxic T cells in HLA-B*2705 transgenic CBA (H-2k) mice
✍ Scribed by Wolfgang Kuon; Roland Lauster; Ute Böttcher; Armin Koroknay; Matthias Ulbrecht; Matthias Hartmann; Martina Grolms; Sanja Ugrinovic; Jürgen Braun; Elisabeth H. Weiss; Joachim Sieper
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 1018 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0004-3591
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✦ Synopsis
Objective. The association of reactive arthritis (ReA) with HLA-B27 and the presence of bacterial antigen in joints with ReA suggest that bacterial peptides might be presented by the HLA-B27 molecule and thus stimulate CD8 T cells. This study was performed to investigate the B27-restricted cytotoxic T lymphocyte (CTL) response to Chlamydia trachomatis, using the model of HLA-B27 transgenic mice.
Methods. CBA (H-2k) mice homozygous for HLA-B*2705 and human &-microglobulin expression were immunized with C trachomatis or with the chlamydia1 57-kd heat-shock protein (hsp57) coupled to latex beads. Cytotoxicity of lymphocytes from in vivo-primed transgenic mice was tested against C trachomatisinfected targets. Blocking experiments were performed with monoclonal antibodies (MAb) against class I major histocompatibility complex molecules.
Results. A Chlamydia-specific lysis of both B27transfected and nontransfected target cells was observed. This response could be inhibited by anti-B27 and anti-H2 MAb. CTL from mice immunized with hsp57 were not able to lyse Chlamydia-infected target cells, and Chlamydia-specific CTL could not destroy targets loaded with hsp57.