Reagents For The Selective Immobilization Of Oligonucleotides On Solid Supports
โ Scribed by Kvach, Maksim V.; Prokhorenko, Igor A.; Ustinov, Alexey V.; Gontarev, Sergei V.; Korshun, Vladimir A.; Shmanai, Vadim V.
- Book ID
- 120508339
- Publisher
- Taylor and Francis Group
- Year
- 2007
- Tongue
- English
- Weight
- 329 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0732-8311
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Linker phosphoramidite reagents containing a protected nucleoside with a cleavable 3%-ester linkage to either succinic acid, diglycolic acid, or hydroquinone-O,O%-diacetic acid (Q-Linker) allow the 3%-terminal nucleoside of an oligonucleotide sequence to be attached to underivatized 'Universal' amin
Very fast (4 sec) attachment of nucleosides to CPG supports, via succinyl or QDA linkers, is possible with either uronium (HBTU, HATU, HBPyU, HBPipU) or phospbonium (BOP, PyBOP ยฎ, Py-BroP, BroP) coupling reagents and automated derivatization of CPG and polystyrene supports just prior to oligonucleot