Reaction of pyridoxal 5′-phosphate with TRIS
✍ Scribed by Michael D. Davis; Dale E. Edmondson; Donald B. McCormick
- Publisher
- Springer Vienna
- Year
- 1982
- Tongue
- English
- Weight
- 273 KB
- Volume
- 113
- Category
- Article
- ISSN
- 0026-9247
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📜 SIMILAR VOLUMES
X ## Ž . The kinetics of the reaction between pyridoxal 5 -phosphate PLP and hydrazine in aqueous solution at a variable pH and a constant strength of 0.1 M was studied spectrophotometrically. The rate constants of formation and hydrolysis of the resulting Schiff base and its stability were also
A new and very sensitive fiuorometric method for the determination of pyridoxal and pyridoxal 5'-phosphate is reported. The specificity is based on the reductive amination of pyridoxal and its Y-phosphate with methyl anthranilate and sodium cyanoborohydride at pH 4.5 to 5.0. Separation of the highly