Thermodynamics properties of pyridoxal 5 phosphate
✍ Scribed by G. Echevarria; M. A. García del Vado; F. García Blanco; M. Menéndez; J. Laynez
- Publisher
- Springer US
- Year
- 1986
- Tongue
- English
- Weight
- 217 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0095-9782
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A new and very sensitive fiuorometric method for the determination of pyridoxal and pyridoxal 5'-phosphate is reported. The specificity is based on the reductive amination of pyridoxal and its Y-phosphate with methyl anthranilate and sodium cyanoborohydride at pH 4.5 to 5.0. Separation of the highly
Markedly increased circulating concentrations of pyridoxal-5'-phosphate (PLP) were found in each of 14 patients representing all clinical forms of bypophosphatasia, an inborn error characterized by deficient activity of the tissue-nonspecific (bone/liver/kidney) isoenzyme of alkaline phosphatase (AP