𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Fluorometric assay of pyridoxal and pyridoxal 5′-phosphate

✍ Scribed by M.S. Chauhan; K. Dakshinamurti

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
517 KB
Volume
96
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A new and very sensitive fiuorometric method for the determination of pyridoxal and pyridoxal 5'-phosphate is reported. The specificity is based on the reductive amination of pyridoxal and its Y-phosphate with methyl anthranilate and sodium cyanoborohydride at pH 4.5 to 5.0. Separation of the highly fluorescent methyl-N-pyridoxyl anthranilate was achieved by a combination of column and thin-layer chromatography on silica gel. This method has been applied to the assay of pyridoxal and pyridoxal Y-phosphate in serum.

📜 SIMILAR VOLUMES

Enzymatic fluorometric assay for plasma
Enzymatic fluorometric assay for plasma pyridoxal 5′-phosphate
✍ Henry C. Poon; Bonnie M. Schmidt 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 369 KB

An enzymatic fluorometric assay to quantitate plasma pyridoxal 5'-phosphate (PLP) is described. PLP is preincubated for 30 min with purified tyrosine decarboxylase apoenzyme (TDA) in acetate buffer and is then incubated with L-tyrosine for 60 min. The decarboxylated metabolite, tyramine, is extracte

Pyridoxal-5′-phosphate and alkaline phos
Pyridoxal-5′-phosphate and alkaline phosphatase
✍ Lawrence Lumeng 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 287 KB

Markedly increased circulating concentrations of pyridoxal-5'-phosphate (PLP) were found in each of 14 patients representing all clinical forms of bypophosphatasia, an inborn error characterized by deficient activity of the tissue-nonspecific (bone/liver/kidney) isoenzyme of alkaline phosphatase (AP