An enzymatic fluorometric assay to quantitate plasma pyridoxal 5'-phosphate (PLP) is described. PLP is preincubated for 30 min with purified tyrosine decarboxylase apoenzyme (TDA) in acetate buffer and is then incubated with L-tyrosine for 60 min. The decarboxylated metabolite, tyramine, is extracte
An improved apotryptophanase assay for pyridoxal phosphate
โ Scribed by Betty E. Haskell; Esmond E. Snell
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 584 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0003-2697
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