rctB mutations that increase copy number of Vibrio cholerae oriCII in Escherichia coli
✍ Scribed by Koch, Birgit; Ma, Xiaofang; Løbner-Olesen, Anders
- Book ID
- 121526437
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 760 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0147-619X
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A temperature shift-up accompanied by a reduction in RNA polymerase activity in Escherichia coli causes an increased rate of initiation leading to a 1.7- to 2.2-fold increase in chromosome copy number. A temperature shift-up without a reduction in polymerase activity induces only a transient non-sch
We describe mutants of Escherichia coli that decrease the plasmid copy number of pBR322 derivatives. One mutant was partially characterized genetically and its mutation, designated pcnB for plasmid copy number, was mapped to approximately 3 rain on the E. coli chromosome. This locus is distinct from