Evidence that a system similar to the recA system of Escherichia coli exists in Vibrio cholerae

✍ Scribed by Ghosh, R. K. ;Siddiqui, K. A. I. ;Mukhopadhyay, G. ;Ghosh, Amit

• Publisher: Springer
• Year: 1985
• Tongue: English
• Weight: 297 KB
• Volume: 200
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00425728

✦ Synopsis

Two lines of evidence suggest that a gene analogous to the recA gene of Escherichia coli exists in Vibrio cholerae and that its product serves a proteolytic function in the SOS response. Firstly, Southern blot hybridization using the recA gene of E. coli as a probe revealed a genomic sequence in V. cholerae which hybridized with the probe. Secondly, the SOS-like response in V. cholerae (as measured by beta phage induction) triggered by DNA damaging agents like Furazolidone could be blocked by Antipain, a protease inhibitor known to inhibit RecA protease action in E. coll. Maximal blocking effect of Antipain on beta phage induction occurred at 1 mM. At this concentration neither the viability of the host bacterium nor the lyric growth of a clear plaque mutant of the phage was affected by Antipain.