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Rapid resolution of 2,4-dinitrophenyl-dl-amino acids on a native cellulose column

✍ Scribed by T. Fukuhara; S. Yuasa

Book ID
104144480
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
324 KB
Volume
411
Category
Article
ISSN
1873-3778

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✦ Synopsis

The biochemical racemizations of amino acids in organisms which occur with aginglp4 and carcinogenesis5, and the geochemical racemizations of free and/or bound amino acids with diagenesi9, have been investigated. These studies require a handy technique for observing the racemization processes. Although methods for separating ' DL-amino acids have been reported -lo, they are not suitable for our purpose from the viewpoints of cost-performance, sensitivity and ease of operation.

The resolution of 2,4-dinitrophenyl(DNP)-DL-amino acids on a native cellulose column has been reported using conventional liquid chromatography1 l. We described here the resolution of sixteen DNP-DL-amino acids by high-performance liquid chromatography (HPLC) and its application to biochemical and geochemical studies.

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