A convenient, rapid method for the resolution of enantiomeric amino acids using chiral phases on stainless-steel capillary gas chromatographic columns
✍ Scribed by Grant Gill Smith; David M. Wonnacott
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 540 KB
- Volume
- 109
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
An improved method is described for the resolution of enantiomeric isopropyl esters of N-trifluoroacetyl-u-amino acids of nonbasic amino acids using N-docosanoyl-L-valyl-rbutylamide and N-octadecanoyl-L-valyl-t.-valine cyclohexyl ester as mixed chiral phases on 150-ft stainless-steel capillary columns. Enantiomers of Ala, Vat. Be. Leu, Ser. Thr. Asp. Met, Glu. and Phe are resolved in I05 min. This method avoids the fractionation problems and high costs encountered with the diastereometric method and difficulties and costs encountered in loading and maintaining glass capillary columns. It is particularly useful for studies involving a large number of resolutions as in a study of the kinetics of racemization of amino acids.