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Radiochromatographic resolution of [14C]DL-tryptophan, [14C]DL-phenylalanine and [35S]DL-methionine on a cellulose column

✍ Scribed by M. Isoyama; T. Fukuhara; M. Tanaka; A. Shimada; S. Yuasa

Publisher: Elsevier Science
Year: 1989
Weight: 236 KB
Volume: 40
Category: Article
ISSN: 0883-2889
DOI: 10.1016/0883-2889(89)90219-0

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✦ Synopsis

[14C]

DL-phenylalanine and [35S]DL-methionine were resolved using cellulose column chromatography. The assignment for the resolved enantiomers was carried out by means of co-chromatography with non-labeled oL-amino acids after modification with fluorodinitrobenzene. The optical purity of the enantiomers was estimated to be greater than 99%. The resolved enantiomers were provided for bioassay, showing that the enantiomer was biochemically active.

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