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Rapid purification of a restriction endonuclease fromEscherichia coliRY13

✍ Scribed by R. S. Mehra; V. P. Malhotra; G. W. Rembhotkar

Book ID
104646503
Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
338 KB
Volume
7
Category
Article
ISSN
0951-208X

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✦ Synopsis

A two step method for the purification of restriction endonuclease Eco RI was developed. The first step involved the purification of the enzyme on Cibacron Blue-F3GA-agarose column, followed by a hydroxyapatite column. The enzyme was homogeneous on SDS-PAGE and completely free from contaminating nucleases and phosphatases, and can be used for direct DNA hydrolysis.

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