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A restriction endonuclease map of Streptomyces phage VWB

✍ Scribed by Anné, Jozef ;Verheyen, Patrick ;Volckaert, Guido ;Eyssen, Hendrik

Publisher: Springer
Year: 1985
Tongue: English
Weight: 181 KB
Volume: 200
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00425741

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✦ Synopsis

A physical map of the actinophage VWB has been constructed using the restriction endonucleases BglII, ClaI, EcoRI, EcoRV, HindIII, KpnI and SphI. Phage VWB, genome size 47.3 kb, propagates on Streptomyces venezuelae, and it can also lysogenise this species. The three BglII-generated fragments of VWB DNA were cloned in pBR322, and subsequently mapped. In this manner the restriction map of the VWB phage genome was constructed.

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