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A detailed restriction endonuclease cleavage map of rat liver mitochondrial DNA

✍ Scribed by Horst Feldmann; Rüdiger Großkopf


Publisher
Springer-Verlag
Year
1979
Tongue
English
Weight
603 KB
Volume
1
Category
Article
ISSN
0172-8083

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✦ Synopsis


A restriction endonuclease cleavage map of rat liver mitochondrial DNA is presented for the following enzymes: Xba I, Bgl II, Hae II, Bam HI, Hpa I, Hha I, Bcl I, Hind II, Hind III, Eco RI, Hpa II, Hae III, and Sau 3A. It was derived from complete and partial digestions with these enzymes, double digestions, and redigestions of defined fragments obtained with one enzyme with other restriction enzymes. By the use of these and further enzymes (Taq YI, Alu I) the mitochondrial DNA (ca. 15.6 Kb) can be dissected into a large number of defined fragments.


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