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MboI,ThaI andHinfI endonuclease cleavage maps of the yeast mitochondrial DNA

✍ Scribed by Micheline Wésolowski; Monique Monnerot; Hiroshi Fukuhara

Publisher
Springer-Verlag
Year
1980
Tongue
English
Weight
698 KB
Volume
2
Category
Article
ISSN
0172-8083

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✦ Synopsis

MboI, HinfI and ThaI cleavage maps have been constructed for the region of the mitochondrial DNA from S. cerevisiae where transfer RNA genes are principally located. About 40 cleavage sites have been localized between the C and P genetic markers. The MboI map covers about 50% of the total mitochondrial genome. For constructing maps we have used a series of rho(-) deletion mutants whose mitochondrial DNAs have a typical single deletion structure as judged by previous genetic and physical analyses. The mutant DNAs carry known transfer RNA genes and genetic markers and, therefore, the comparison between genetic and restriction maps has allowed us to localize individual transfer RNA genes within defined physical segments.

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